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Journal / Developmental Biology / Live-Imaging af medfødte immunsystem og…
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Developmental Biology

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JoVE Journal Developmental Biology
Live-Imaging af medfødte immunsystem og præneoplastiske celleinteraktioner Brug en inducerbar Gal4 / UAS Expression System i Larve Zebrafisk Skin
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Live-Imaging af medfødte immunsystem og præneoplastiske celleinteraktioner Brug en inducerbar Gal4 / UAS Expression System i Larve Zebrafisk Skin

Article DOI: 10.3791/52107-v • 08:52 min • February 3rd, 2015
February 3rd, 2015 •
1, 1, 1, 2, 1
1MRC Centre for Inflammation Research, Queen's Medical Research Institute, The University of Edinburgh, 2Department of Cell & Developmental Biology, University College London

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At studere de tidligste begivenheder af præneoplastiske celle progression og medfødte immunforsvar interaktion celle er afgørende at forstå og behandle kræft. Her beskriver vi en metode til betinget fremkalde epitelcelletyper transformationer og den efterfølgende live-billeddannelse af medfødte immunforsvar interaktion celle med HRAS G12V udtrykker hudceller i zebrafisk larver.

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Developmental Biology zebrafisk live billedbehandling hud betinget genekspression KalTA4-ER 4-hydroxytamoxifen HRAS
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