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Journal / Biology / Purifying the Impure: Sequencing Metagenomes and…
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Purifying the Impure
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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Article DOI: 10.3791/52117-v • 11:23 min • December 22nd, 2014
December 22nd, 2014 •
1, 2, 1, 3, 4, 1
1Department of Biology, San Diego State University, 2DOE Joint Genome Institute, 3Department of Molecular, Cellular and Developmental Biology, University of Colorado, 4Department of Pediatrics, School of Medicine, University of Colorado

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Summary
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Using the cystic fibrosis airway as an example, the manuscript presents a comprehensive workflow comprising a combination of metagenomic and metatranscriptomic approaches to characterize the microbial and viral communities in animal-associated samples.

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Metagenomes Metatranscriptomes High-throughput Sequencing DNA Extraction RNA Extraction Viral Communities Microbial Communities Taxonomical Characteristics Community Encoded Functions Cystic Fibrosis Sputum Mucins Neutrophil DNA Unknown Contaminants Human DNA Contamination Metatranscriptomics Protocol MRNA Ribosomal RNA Sequences Microbiome Profiles
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