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JoVE Journal Chemistry
Anvendelse af stopped-flow fluorescens og mærkede nukleotider Analyser ATP Omsætning Cycle of Kinesins
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Anvendelse af stopped-flow fluorescens og mærkede nukleotider Analyser ATP Omsætning Cycle of Kinesins

Article DOI: 10.3791/52142-v • 07:25 min • October 17th, 2014
October 17th, 2014 •
1, 1, 1, 1
1School of Life Sciences, University of Nottingham

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Kinesins er kendetegnet ved nukleotid-afhængig interaktion med mikrotubuli: en cyklus af ATP omsætning koblet til en cyklus af mikrotubulus interaktion. Her beskriver vi protokoller til at analysere kinetikken af ​​enkelte nucleotid-overgange i ATP omsætning cycle kinesin hjælp fluorescensmærkede nukleotider og stoppet-flow-fluorescens.

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Kemi kinesin ATP omsætning mantATP mantADP stoppede flow fluorescens mikrotubuli enzymkinetik nukleotid
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