Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolation and Enrichment of Human Adipose-derived …
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
Journal (Developmental Biology)
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
Journal (Developmental Biology)
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
 

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Article DOI: 10.3791/52181-v • 11:00 min • January 12th, 2015
January 12th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University
* These authors contributed equally

Chapters

Summary
Automatic Translation

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

Tags

Isolation Enrichment Human Adipose-derived Stromal Cells Osteogenesis Bone Marrow-derived Mesenchymal Stromal Cells Stem Cell-based Tissue Engineering Donor Site Morbidity Adipose-derived Stromal Cells ASCs Osteogenic Lineage Cells Tissue Engineering Applications Scaffolds Craniofacial Skeletal Defects Stromal Vascular Fraction Heterogeneous Mixture Surface Marker Profile Osteoprogenitor Cells Critical-sized Calvarial Defects
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter