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Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
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Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Article DOI: 10.3791/52217-v • 11:13 min • January 7th, 2015
January 7th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1,2
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
* These authors contributed equally

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Summary
Automatic Translation

Fat grafting is an essential technique for reconstructing soft tissue deficits. However, it remains an unpredictable procedure characterized by variable graft survival. Our goal was to devise a mouse model that utilizes a novel imaging method to compare volume retention between differing techniques of fat graft preparation and delivery.

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Viability Assessment Human Fat Injection Nude Mice Micro-computed Tomography Lipotransfer Soft Tissue Deficits Fat Grafting Unpredictable Results Graft Survival Animal Model Real-time Analysis Injected Fat Volume Retention Scalp Cut CD-1 Nude Mouse Processed Lipoaspirate Recipient Site Background Contrast Micro-CT Scan Imaging Software Graft Volumes Euthanizing Study Animal Physical Measurement Ex Vivo Biochemical Comparisons Histological Comparisons
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