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Elektroforetisk mobilitet (EMSA) for Studiet af RNA-protein interaktioner
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Elektroforetisk mobilitet (EMSA) for Studiet af RNA-protein interaktioner: Den IRE / IRP Eksempel

Article DOI: 10.3791/52230-v • 12:44 min • December 3rd, 2014
December 3rd, 2014 •
1,2, 1,2, 1,2
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Department of Medicine, McGill University

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Her præsenterer vi en protokol til at analysere RNA / protein-interaktioner. Den elektroforetiske mobilitet (EMSA) er baseret på forskellen migration af RNA / protein-komplekser og fri RNA under nativ gelelektroforese. Ved anvendelse af en radioaktivt mærket RNA-probe, kan RNA / protein komplekser visualiseret ved autoradiografi.

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Biokemi RNA metabolisme mRNA translation posttransskriptionel genregulering mRNA stabilitet IRE IRP1 IRP2 jern metabolisme ferritin transferrin receptor
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