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Måling af mRNA henfaldskurver i
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Måling af mRNA henfaldskurver i

Article DOI: 10.3791/52240-v • 12:21 min • December 13th, 2014
December 13th, 2014 •
1, 1
1Department of Biology, Baylor University

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Steady state-niveau af specifikke mRNA'er er bestemt af hastigheden af ​​syntese og nedbrydning af mRNA. Genom-dækkende mRNA nedbrydning satser eller henfaldet specifikke mRNA'er kan måles ved at bestemme mRNA halveringstider. Denne protokol fokuserer på måling af mRNA henfald satser i Saccharomyces cerevisiae.

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Cellebiologi , MRNA henfald mRNA stabilitet nonsens-medieret mRNA henfald mRNA halveringstid transskription hæmning
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