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JoVE Journal Developmental Biology
Misurare Protein Stabilità in Living embrioni di zebrafish Usando fluorescenza Decay Dopo Fotoconversione (FDAP)
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Misurare Protein Stabilità in Living embrioni di zebrafish Usando fluorescenza Decay Dopo Fotoconversione (FDAP)

Article DOI: 10.3791/52266-v • 09:45 min • January 28th, 2015
January 28th, 2015 •
1, 2, 1, 2
1Department of Molecular and Cellular Biology, Harvard University, 2Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society

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I livelli di proteine ​​nelle cellule e tessuti sono spesso strettamente regolati dal saldo della produzione di proteine ​​e di liquidazione. Usando fluorescenza Decay Dopo Fotoconversione (FDAP), la cinetica di clearance di proteine ​​possono essere sperimentalmente misurati in vivo.

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Biologia dello Sviluppo fluorescenza Decay Dopo Fotoconversione (FDAP) stabilità proteica tasso di liquidazione la microscopia confocale proteine ​​fotoconvertibile Dendra2 embrione zebrafish
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