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Journal / Neuroscience / Laser Capture mikrodissektion - en demonstration…
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Neuroscience

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JoVE Journal Neuroscience
Laser Capture mikrodissektion - en demonstration af Isolering af Individual dopaminneuroner og hele Ventral tegmentalområde
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Laser Capture mikrodissektion - en demonstration af Isolering af Individual dopaminneuroner og hele Ventral tegmentalområde

Article DOI: 10.3791/52336-v • 08:29 min • February 6th, 2015
February 6th, 2015 •
1, 1, 1
1Department of Basic Sciences, Mississippi State University College of Veterinary Medicine

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Isoleringen af ​​individuelle dopaminneuroner eller ventrale tegmentalområde med direkte eller indirekte immunhistokemi påvises ved hjælp af laser capture mikrodissektion. Parametre til isolering af væv fra et objektglas under anvendelse af en infrarød laser og fra membran- slides anvendes en kombination af en infrarød og ultraviolet laser diskuteres.

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Neuroscience Laser capture mikrodissektion dopamin neuron Immunohistokemi Tyrosin hydroxylase Ventral tegmentalområde PEN membran objektglas.
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