Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

纯化野生型和突变CFTR蛋白的功能重建和通道活性测定
 
Click here for the English version

纯化野生型和突变CFTR蛋白的功能重建和通道活性测定

Article DOI: 10.3791/52427-v 09:59 min March 9th, 2015
March 9th, 2015

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

这里描述的是一种快速,有效的方法为纯化的野生型和突变型CFTR蛋白的功能性重组,可以保留活动而此氯离子通道,这是有缺陷的囊性纤维化。从由CFTR介导的重组蛋白脂质体碘流出允许通道活性的研究和小分子的影响。

Tags

生物化学,第97,囊性纤维化,CFTR,纯化,复溶,氯离子通道,通道功能,碘化流出,增强
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter