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Funktionel Opløsning og kanalaktivitet Målinger af renset Vildtype og Mutant CFTR Protein
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Funktionel Opløsning og kanalaktivitet Målinger af renset Vildtype og Mutant CFTR Protein

Article DOI: 10.3791/52427-v • 09:59 min • March 9th, 2015
March 9th, 2015 •
1, 1, 1,2,3
1Programme in Molecular Structure and Function, Hospital for Sick Children, 2Department of Biochemistry, University of Toronto, 3Department of Physiology, University of Toronto

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Beskrevet her er en hurtig og effektiv procedure for funktionel rekonstitution af oprenset vildtype og mutant CFTR-protein, som bevarer aktivitet for denne chloridkanal, som er defekt i cystisk fibrose. Iodid efflux fra rekonstitueret proteoliposomer medieret af CFTR tillader studier af kanalaktivitet og virkningerne af små molekyler.

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Biokemi cystisk fibrose CFTR rensning rekonstituering klorid kanal kanal-funktion iodid efflux potensering
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