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JoVE Journal
Developmental Biology

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基于ES-Cell的编程组合MYH6启动子,选择小鼠心脏起搏器细胞聚集一代
 
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基于ES-Cell的编程组合MYH6启动子,选择小鼠心脏起搏器细胞聚集一代

Article DOI: 10.3791/52465-v 08:52 min February 17th, 2015
February 17th, 2015

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Summary

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本协议描述了如何制作从小鼠多能干细胞(PSC)功能性鼻窦淋巴组织。 T-BOX3(TBX3)表达以及心肌肌球蛋白重链(MYH6)启动抗生素的选择导致了高纯度的起搏细胞聚集。这些“诱导窦房结-机构”(“iSABs”)含有80%以上的起搏细胞,显示高度增加跳动的速度,并能步伐心肌体外

Tags

发育生物学,第96,鼠,多能干细胞(PSC),病态窦房结综合征,iSABs,致窦房机构,心肌,心脏起搏器
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