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JoVE Journal Immunology and Infection
At analysere virkningen af ​​stromacellers om ansættelse af Leukocytter fra Flow
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At analysere virkningen af ​​stromacellers om ansættelse af Leukocytter fra Flow

Article DOI: 10.3791/52480-v • 11:30 min • January 7th, 2015
January 7th, 2015 •
1,2, 1,2, 1,2, 2,3
1School of Clinical and Experimental Medicine, University of Birmingham, 2College of Medical and Dental Sciences, University of Birmingham, 3School of Immunity and Infection, University of Birmingham

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Evne betændt endotel at rekruttere leukocytter fra flow reguleres af mesenkymale stromale celler. Vi beskriver to in vitro modeller med primære humane celler, som kan anvendes til at vurdere neutrofil rekruttering fra flow og undersøge den rolle, som mesenchymale stromale celler spiller i reguleringen af denne proces.

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Immunology endotelceller leukocytter mesenchymal stromaceller mesenchymale stamceller co-kultur adhæsion inflammation rekruttering flow baseret adhæsion assay Ibidi mikroobjektglas neutrofil
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