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Targeted DNA Methylation Analysis by Next-generation Sequencing

Article DOI: 10.3791/52488-v • 08:42 min • February 24th, 2015
February 24th, 2015 •
1, 1, 1,2
1Department of Physiology, University of Oklahoma College of Medicine, 2Department of Geriatric Medicine, University of Oklahoma College of Medicine

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Summary
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Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.

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Targeted DNA Methylation Analysis Next-generation Sequencing Epigenetic Processes Gene Expression Control DNA Methylation Cytosine Residues Epigenetic Studies High-throughput Methods Quantitatively Accurate Methods Next-generation Sequencing Technologies Epigenomics Genomic Methodologies Transcriptomic Methodologies Bisulfite Amplicon Sequencing (BSAS) Bisulfite Conversion Targeted Amplification Transposome-mediated Library Construction Benchtop NGS Analysis Of Targeted Regions Research Groups Molecular Biology Skills Absolute Quantitation Of Cytosine Methylation
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