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Journal / Immunology and Infection / Onderzoekt mestcel secretiegranula; Biosynthese…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Onderzoekt mestcel secretiegranula; Biosynthese van exocytose
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
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Onderzoekt mestcel secretiegranula; Biosynthese van exocytose

Article DOI: 10.3791/52505-v • 16:01 min • January 26th, 2015
January 26th, 2015 •
1,2, 3, 2, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

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Het doel van het huidige protocol was om een ​​methode die functioneel genomische analyses van mestcel secretie zal ontwikkelen. Het protocol is gebaseerd op kwantitatieve beoordeling van de release van een tl-reporter-gen cotrasfected met het gen van interesse en real-time analyses van de morfologie van de secretoire korrel's.

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Immunologie Mast cellen exocytose secretoire korrels live cell imaging Rab5 reporter-gen confocale beeldvorming transfectie
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