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Journal / Developmental Biology / Dobbelt mærkning af neurale crest celler og…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Dobbelt mærkning af neurale crest celler og blodkar i kyllingembryoner ved hjælp af ChickGFP Neural Tube Poding og Carbocyanine Dye DiI Injection
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Dobbelt mærkning af neurale crest celler og blodkar i kyllingembryoner ved hjælp af ChickGFP Neural Tube Poding og Carbocyanine Dye DiI Injection

Article DOI: 10.3791/52514-v • 09:57 min • May 28th, 2015
May 28th, 2015 •
1,2, 1, 1,3
1Birth Defects Research Centre, UCL Institute of Child Health, 2Blizard Institute, Centre for Digestive Diseases, Queen Mary University of London, Barts and The London School of Medicine and Dentistry, 3Department of Clinical Genetics, Erasmus University Medical Center, Rotterdam

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Her rapporterer vi dobbelt mærkning af neurale crest celler og blodkar ved hjælp af chickGFP neuralrør intraspecies podning kombineret med intra-vaskulære DiI injektion. Denne eksperimentelle teknik giver os mulighed for samtidig at visualisere og studere udviklingen af det NCC-afledte (enteriske) nervesystem og det vaskulære system under organogenese.

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Udviklingsbiologi Problem 99 Intraspecies podning kimære neuralrør karmaleri carburonfarve vaskulært netværk transgen GFP kylling neurale crest celler enterisk nervesystem
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