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March 30, 2015
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Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.
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Cite this Article
Crannell, Z. A., Rohrman, B., Richards-Kortum, R. Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control. J. Vis. Exp. (97), e52620, doi:10.3791/52620 (2015).
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