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测定冷灵敏度和适应小鼠一种简单廉价的方法
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
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测定冷灵敏度和适应小鼠一种简单廉价的方法

DOI:
10.3791/52640-v

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08:35 min

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March 17, 2015

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, , ,
1MSTP, Neuroscience Program,Washington University in St. Louis, 2Washington University Pain Center, Department of Anesthesiology,Washington University in St. Louis

Chapters

  • 00:05Title
  • 01:14Preparing the Testing Plate and Enclosures
  • 02:03Performing the Cold Plantar Assay (CPA) at Fixed Temperatures
  • 05:10Testing the Mice During Cold Adaptation
  • 05:54Results: Measuring Analgesia and Hypersensitivity Using the Cold Planar Assay
  • 08:00Conclusion

Summary

Automatic Translation

Automatic Translation

The Cold Plantar Assay (CPA) measures cold responsiveness between 30 °C and 5 °C, and can also measure cold adaptation. This protocol describes how to use the CPA to measure cold hypersensitivity, analgesia, and adaptation in mice.

Tags

Cold SensitivityCold HypersensitivityCold Plantar AssayCold AdaptationThermal ResponseCold AnalgesiaCold NociceptionMouse BehaviorTemperature Sensitivity

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