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Journal / Immunology and Infection / Utnytte Antigen kapsidbindende innlemmelse…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Utnytte Antigen kapsidbindende innlemmelse strategi for utvikling av Adenovirus Serotype 5-vectored Vaksine tilnærminger
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Utnytte Antigen kapsidbindende innlemmelse strategi for utvikling av Adenovirus Serotype 5-vectored Vaksine tilnærminger

Article DOI: 10.3791/52655-v • 13:36 min • May 6th, 2015
May 6th, 2015 •
1, 1, 1, 1,2
1Department of Medicine, University of Alabama at Birmingham, 2Center for AIDS Research, University of Alabama at Birmingham

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Her presenterer vi en protokoll for å generere en proof-of-prinsippet toverdig adenovirus type 5 (Ad5) vektor Ad5 / H5-HVR1-KWAS-HVR5-His 6 ved å utnytte Antigen kapsidbindende innlemmelse strategi. Denne vektor ble vist å oppvise kvalitativt kondisjon, evnen til å unnslippe Ad5-positive sera in vitro, og antigenisiteten og immunogenisiteten til de inkorporerte antigener.

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Immunologi Antigen kapsidbindende innlemmelse strategi transgen metode Adenovirus (Ad) vaksine kapsidproteiner dual modifikasjon pre-eksisterende immunitet (PEI)
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