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Immunology and Infection

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Biolistisk transformation af en fluorescerende Tagged gen i den opportunistiske svampepatogenet
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Biolistisk transformation af en fluorescerende Tagged gen i den opportunistiske svampepatogenet

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistisk transformation er en metode, der anvendes til at generere en stabil integration af DNA i genomet af de opportunistiske patogen Cryptococcus neoformans gennem homolog rekombination. Vi vil demonstrere biolistisk transformation af en konstruktion, som har genet, der koder acetat kinase fusioneret til fluorescerende tag mCherry i C. neoformans.

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Molekylærbiologi genafbrydelse, acetat kinase overlap PCR fluorescens
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