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Journal / Immunology and Infection / Biolistischen Transformation einer Leuchtstoff…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Biolistischen Transformation einer Leuchtstoff Tagged-Gens in den Opportunistic Pilzpathogenentwicklung
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Journal (Immunology and Infection)
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
Journal (Immunology and Infection)
Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
 
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Biolistischen Transformation einer Leuchtstoff Tagged-Gens in den Opportunistic Pilzpathogenentwicklung

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistische Transformation ein Verfahren verwendet werden, um eine stabile Integration der DNA in das Genom der opportunistisches Pathogen Cryptococcus neoformans durch homologe Rekombination zu erzeugen. Wir biolistische Transformation eines Konstrukts, das den kodierenden Gens Acetatkinase zur Fluoreszenzmarkierung mCherry in C. neoformans fusioniert hat demonstrieren.

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Molecular Biology Ausgabe 97 Gen-Unterbrechung, Biolistischen Lieferung Acetatkinase überlappende PCR Fluoreszenz-
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