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Immunology and Infection

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JoVE Journal Immunology and Infection
Trasformazione Biolistic di una fluorescente Tagged Gene nel Opportunistic Fungal patogeni
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Journal (Immunology and Infection)
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
Journal (Immunology and Infection)
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
 
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Trasformazione Biolistic di una fluorescente Tagged Gene nel Opportunistic Fungal patogeni

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Trasformazione Biolistic è un metodo utilizzato per generare stabile integrazione del DNA nel genoma delle opportunistiche neoformans patogeno Cryptococcus tramite ricombinazione omologa. Dimostreremo trasformazione biolistica di un costrutto, che ha l'acetato chinasi gene codificante fuso al tag mCherry fluorescente in C. neoformans.

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Biologia Molecolare interruzione gene, Consegna biolistic chinasi acetato sovrapposizione PCR fluorescenza
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