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Journal / Immunology and Infection / Fırsatçı mantar patojenlere bir Floresan etiketli …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Fırsatçı mantar patojenlere bir Floresan etiketli geninin biyolistik transformasyonu
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Fırsatçı mantar patojenlere bir Floresan etiketli geninin biyolistik transformasyonu

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biyolistik dönüşüm homolog yeniden bağlama aracılığıyla fırsatçı patojen Cryptococcus neoformans genomuna DNA'nın kararlı bütünleşmesinin oluşturmak için kullanılan bir yöntemdir. Biz C.neoformans içine floresan etiket mCherry kaynaşmış kodlayan gen asetat kinaz olan bir yapı, biolistik dönüşümü gösterecektir.

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Moleküler Biyoloji Sayı 97 gen bozulması, Biyolistik asetat kinaz örtüşme PCR floresan
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