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Immunology and Infection

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JoVE Journal Immunology and Infection
Semplificato umani neutrofili extracellulari Traps (NET) Isolamento e Manipolazione
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A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
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Semplificato umani neutrofili extracellulari Traps (NET) Isolamento e Manipolazione

Article DOI: 10.3791/52687-v • 09:43 min • April 16th, 2015
April 16th, 2015 •
*1, *1, 1, 1, 1
1LD MacLean Surgical Research Laboratories, Department of Surgery, McGill University
* These authors contributed equally

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Nella seguente protocollo, si descrive un modo molto semplice per isolare trappole neutrofili extracellulari (NET) da sangue umano intero utilizzando reagenti facilmente disponibili. Abbiamo poi mostrato come i NET isolati possono essere utilizzati in un'adesione test in vitro con cellule tumorali.

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Immunologia neutrofili trappole extracellulari dei neutrofili infiammazione i batteri le infezioni il cancro la metastasi adesione
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