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Isolamento di cellule umane endoteliali linfatico da Multi-parametro fluorescenza-attivato cell sorting

Article DOI: 10.3791/52691-v • 07:36 min • May 1st, 2015
May 1st, 2015 •
1,2, 1,3, 1, 1,2,3, 1,2, 1,2,3
1Murdoch Childrens Research Institute, The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

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L'obiettivo di questo protocollo è quello di isolare le cellule endoteliali linfatiche rivestono i vasi cisti-come malformazione linfatica umani e prepuzi utilizzando cellule fluorescenza attivate (FACS). Coltura delle cellule e successiva espansione di queste cellule permette un nuovo livello di sofisticazione sperimentale per studi genetici, proteomica, funzionali e differenziazione cellulare.

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Medicina , citometria a flusso ordinamento, marcatori di superficie delle cellule
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