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Isolering av human lymfatisk endotelceller ved Multi-parameter fluorescens-aktivert cellesortering
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Isolering av human lymfatisk endotelceller ved Multi-parameter fluorescens-aktivert cellesortering

Article DOI: 10.3791/52691-v • 07:36 min • May 1st, 2015
May 1st, 2015 •
1,2, 1,3, 1, 1,2,3, 1,2, 1,2,3
1Murdoch Childrens Research Institute, The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

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Målet med denne protokollen er å isolere lymfatiske endotelceller lining menneskelige lymfesystemet misdannelsescystelignende fartøy og forhud bruker fluorescensaktivert celle sortering (FACS). Påfølgende celledyrking og utvidelse av disse cellene tillater et nytt nivå av eksperimentell raffinement for genetiske, proteomikk, funksjonelle og celle differensiering studier.

Tags

Medisin , flowcytometrisk sortering, celleoverflatemarkører
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