A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Section of Urology, Department of Surgery,The University of Chicago, 2Department of Pathology,Robert Wood Johnson Medical School, 3Campbell Family Institute for Breast Cancer Research, Princess Margaret Cancer Centre,University Health Network, 4Department of Laboratory Medicine and Pathobiology, Campbell Family Institute for Breast Cancer Research, Princess Margaret Cancer Centre,University of Toronto, University Health Network, 5Departments of Medicine, Pharmacology, and Cancer Biology,Duke University Medical Center
Chapters
- 00:05Title
- 02:25Intraperitoneal Injection of Cells
- 04:08Identification and Isolation of Peritoneal Fat Depots
- 06:14Quantitation of Cancer Cell Localization to Milky Spots Using Flow Cytometry
- 08:33Ex vivo Co-culture of the Mouse Omentum with SKOV3ip.1-GFP Cells
- 10:06Results: Analysis of Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose
- 12:06Conclusion
We outline a protocol that implements both in vivo and ex vivo approaches to study ovarian cancer colonization of peritoneal adipose tissues, particularly the omentum. Furthermore, we present a protocol to quantitate and analyze immune cell-structures in the omentum known as milky spots, which promote metastases of peritoneal adipose.
