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JoVE Journal Bioengineering
Papirbaserte Enheter for isolering og karakterisering av Ekstracellulær Blemmer
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Papirbaserte Enheter for isolering og karakterisering av Ekstracellulær Blemmer

Article DOI: 10.3791/52722-v • 11:53 min • April 3rd, 2015
April 3rd, 2015 •
1, 1, 2, 1
1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Taichung Veterans General Hospital

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Denne protokollen detaljer en metode for å isolere ekstracellulære vesikler (EVS), små membran partikler frigjøres fra celler, fra så lite som 10 pl serumprøver. Denne tilnærmingen omgår behovet for ultrasentrifugering, krever bare noen få minutter av analysen tid, og muliggjør isoleringen av el-biler fra prøver av begrensede volumer.

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Bioteknologi ekstracellulære vesikler exosomes cellulose papir MicroFluidics papir ELISA vandig humor kjemisk konjugering
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