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JoVE Journal Developmental Biology
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Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Article DOI: 10.3791/52726-v • 07:05 min • April 9th, 2015
April 9th, 2015 •
1,2, 1,2, 1,2,3
1Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 2Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, 3The Cyrus Tang Hematology Center, Soochow University

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Summary
Automatic Translation

The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.

Tags

Isolation Cryopreservation Neonatal Rat Cardiomyocytes Cell Culture Proliferation Culturing Cryoprotectant Dimethyl Sulfoxide (DMSO) Freezing/thawing Methods Sensitivity Viable Cells Phenotype Immunocytochemistry Staining α-sarcomeric Actinin Spontaneous Contraction Cell Viability Flexibility Animal Reduction
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