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Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
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Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein

Article DOI: 10.3791/52752-v • 12:01 min • May 30th, 2015
May 30th, 2015 •
1, 1, 2, 3, 4, 5, 5, 2, 1
1Neurodegenerative Diseases Unit, ANSES - French Agency for Food, Environmental and Occupational Health & Safety, 2Indicia Production, 3AJ Roboscreen GmbH, 4Epidemiology Unit, ANSES - French Agency for Food, Environmental and Occupational Health & Safety, 5Experimental Facilities, ANSES - French Agency for Food, Environmental and Occupational Health & Safety

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Summary
Automatic Translation

An ELISA offering a novel quantitative approach is described. It specifically detects disease-associated α-synuclein (αSD) in a transgenic mouse model (M83) of synucleinopathy using several antibodies against either the Ser129 phosphorylated αS form or the C-terminal part of the protein.

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Disease-associated α-synuclein A53T Mutated α-synuclein ELISA Transgenic Mice Synucleopathies Molecular Analysis Central Nervous System Caudal Brain Regions Spinal Cord Antibody Ser129 Phosphorylated αS Western Blot Immunohistochemistry
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