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Immunology and Infection

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JoVE Journal Immunology and Infection
Analyse des fonctions des mastocytes in vivo à l’aide de sourisknock-inde mastocytes
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Analyse des fonctions des mastocytes in vivo à l’aide de sourisknock-inde mastocytes

Article DOI: 10.3791/52753-v • 09:07 min • May 27th, 2015
May 27th, 2015 •
1, 1, 1, 1, 1,2, 1
1Department of Pathology, Stanford University School of Medicine, 2Department of Microbiology & Immunology, Stanford University School of Medicine

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Nous décrivons une méthode pour la génération des mastocytes dérivés in vitro, leur greffe en souris mastocytaires-déficientes, et l’analyse du phénotype, des nombres et de la distribution des mastocytes engrafted à différents emplacements anatomiques. Ce protocole peut être utilisé pour évaluer les fonctions des mastocytes in vivo.

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Immunologie et infection numéro 99,c-kit facteur de cellules souches FcεRI immunoglobuline E modèle murin transfert adoptif immunologie allergie
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