Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Comet Assay as an Indirect Measure of Systemic…
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
Comet Assay as an Indirect Measure of Systemic Oxidative Stress
Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
Journal (Biology)
Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
Journal (Biology)
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
 

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Article DOI: 10.3791/52763-v • 08:23 min • May 22nd, 2015
May 22nd, 2015 •
1,2, 1,2, 1, 1, 1
1Department of Ophthalmology, University of Basel, 2Department of Biomedicine, University of Basel

Chapters

Summary
Automatic Translation

Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative stress.

Tags

Comet Assay Systemic Oxidative Stress Eukaryotic Organisms Oxygen Molecule Reactive Oxidative Species (ROS) Repair Capacity Oxidative Stress Measurement Cell Gel Electrophoresis DNA Breaks DNA Damage
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter