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Comet assay som et indirekte mål for Systemisk oxidativt stress
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Comet assay som et indirekte mål for Systemisk oxidativt stress

Article DOI: 10.3791/52763-v • 08:23 min • May 22nd, 2015
May 22nd, 2015 •
1,2, 1,2, 1, 1, 1
1Department of Ophthalmology, University of Basel, 2Department of Biomedicine, University of Basel

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Comet assay måler DNA-brud, fremkaldt af forskellige faktorer. Hvis holdes konstant faktorer (undtagen oxidativ stress) forårsager DNA-skader, mængden af ​​DNA-skader er en god indirekte parameter for oxidativ stress. Målet med denne protokol er at bruge komet assay til indirekte måling af oxidativ stress.

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Molekylærbiologi Comet assay analyse Single-celle gelelektroforese DNA pauser Oxidative stress
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