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JoVE Journal
Biology

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基于海拉细胞自由表达系统中的表达
 
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基于海拉细胞自由表达系统中的表达

Article DOI: 10.3791/52772
June 10th, 2015

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Summary June 10th, 2015

Please note that all translations are automatically generated.

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基于细胞系统疟疾蛋白的表达仍然具有挑战性。我们证明两步并一步IVT( 体外翻译)无细胞表达系统从HeLa细胞表达的重组疟疾蛋白质棒状体。我们使用的Ni树脂基于亲和性的纯化系统纯化棒状体蛋白质。

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