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Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds
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Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Article DOI: 10.3791/52792-v • 10:16 min • June 6th, 2015
June 6th, 2015 •
1, 2, 2, 1, 3, 1, 1
1Department of Radiation Oncology and Experimental Cancer Research, University of Ghent, 2Department of Sustainable Organic Chemistry and Technology, University of Ghent, 3Department of Chemistry, University of Delhi

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Here, we present a protocol to study the invasion of tumor cells into living normal tissue fragments in three dimensions. This organ culture technique is mainly applied to test potentially anti-invasive drugs in vitro.

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Chick Heart Assay Invasiveness Of Cancer Cells Anti-invasive Compounds Tumor Invasion Test Compounds Organ Culture Method Three-dimensional Interaction Histological Analysis Hematoxylin-eosin Staining Immunohistochemical Staining Stromal Host Elements Extracellular Matrix Components
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