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Journal / Developmental Biology / Isolierung und Charakterisierung von…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolierung und Charakterisierung von Satellitenzellen aus Ratte Kopf Branchiomeric Muscles
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Isolierung und Charakterisierung von Satellitenzellen aus Ratte Kopf Branchiomeric Muscles

Article DOI: 10.3791/52802-v • 07:37 min • July 20th, 2015
July 20th, 2015 •
1, 2, 3, 1, 1, 1
1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2Department of Biological Structure, University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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Dieses Protokoll beschreibt die Isolierung von Satellitenzellen aus branchiomeric Kopf Muskeln über ein 9 Wochen alten Ratte. Die Muskeln aus unterschiedlichen Kiemenbögen. Anschließend werden die Satellitenzellen auf einem Spotlackierung von Millimetergröße, um ihre Differenzierung zu untersuchen kultiviert. Dieser Ansatz vermeidet die Expansion und Passagieren von Satellitenzellen.

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Developmental Biology Ausgabe 101 Kopf Muskeln levator veli palatini Muskel M. digastricus M. masseter Satellitenzellen Isolation Primärzellen Gaumenspalte regenerative Medizin Tissue Engineering Stammzellen Differenzierung Muskelfasern
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