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Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
JoVE Journal
Biology
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JoVE Journal Biology
Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

20,597 Views

12:58 min

June 18, 2015

DOI:

12:58 min
June 18, 2015

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Summary

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Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

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