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Proteína entrega directa de células de mamíferos Utilización de Células permeable Cys

Article DOI: 10.3791/52814-v • 11:24 min • March 25th, 2015
March 25th, 2015 •
*1, *1,2
1Departments of Chemistry and Cell and Molecular Biology, The Scripps Research Institute, 2Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
* These authors contributed equally

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Dominios de dedos de zinc son células permeable intrínseca y capaz de mediar la entrega de proteínas en una amplia gama de tipos de células de mamíferos. Aquí, se presenta un protocolo detallado paso a paso para la implementación de la tecnología zinc-finger para la entrega de proteínas intracelulares.

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Biología Molecular Número 97 la entrega de proteínas péptidos de penetración celular zinc-finger dominio de transducción de proteína biología química biología molecular
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