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Journal / Immunology and Infection / Metoder for å øke følsomheten av High Resolution…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Metoder for å øke følsomheten av High Resolution Melting Enkelt nucleotide polymorphism Genotyping i Malaria
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Metoder for å øke følsomheten av High Resolution Melting Enkelt nucleotide polymorphism Genotyping i Malaria

Article DOI: 10.3791/52839-v • 10:27 min • November 10th, 2015
November 10th, 2015 •
1,2, 2, 2, 3, 2,5, 1, 2,4
1Department of Organismic and Evolutionary Biology, Harvard University, 2Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 3Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, 4School of Nursing and Health Sciences, Simmons College, 5Institute of Infectious Diseases, Broad Institute

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Mens høy oppløsning smelte analyse gir evnen til å differensiere mellom enkelt-nukleotid polymorfismer i en heterogen populasjon, kan mutant allel forsterkning forspenning øke dens evne til å påvise tilstedeværende alleler ved relativt lave prosentandeler i en prøve. Denne protokollen beskriver forbedringer som forbedrer følsomheten av høy oppløsning smelteanalyse.

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Immunologi Plasmodium malaria utrydding motstand høy oppløsning smelte analyse genotyping
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