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JoVE Journal Developmental Biology
Erzeugung von induzierten pluripotenten Stammzellen aus Gefrorene Buffy Coats mit Non-Integration episomalen Plasmiden
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Erzeugung von induzierten pluripotenten Stammzellen aus Gefrorene Buffy Coats mit Non-Integration episomalen Plasmiden

Article DOI: 10.3791/52885-v • 10:52 min • June 5th, 2015
June 5th, 2015 •
*1, *1, 1, 1, 2, 2, 3, 1, 1, 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
* These authors contributed equally

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Pluripotenten Stammzellen (iPS) Quelle eines patientenspezifischen Geweben für die klinische Anwendung und Grundlagenforschung. Hier präsentieren wir ein detailliertes Protokoll für die menschliche periphere mononukleäre Blutzellen (PBMNC) von gefrorenen Buffy Coats in virale freien iPSCs mit nicht-integrierenden episomalen Plasmiden erhalten neu zu programmieren.

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Developmental Biology Ausgabe 100 Stammzellenbiologie Zellbiologie Molekularbiologie induzierte pluripotente Stammzellen peripheren mononukleären Blutzellen Neuprogrammierung episomalen Plasmiden.
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