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JoVE Journal Developmental Biology
Generering av inducerade pluripotenta stamceller från Frozen Buffy Coats använder icke-integrerande Episomalt plasmider
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Generering av inducerade pluripotenta stamceller från Frozen Buffy Coats använder icke-integrerande Episomalt plasmider

Article DOI: 10.3791/52885-v • 10:52 min • June 5th, 2015
June 5th, 2015 •
*1, *1, 1, 1, 2, 2, 3, 1, 1, 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
* These authors contributed equally

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Inducerade pluripotenta stamceller (iPSCs) utgör en källa till patientspecifika vävnader för kliniska tillämpningar och grundforskning. Här presenterar vi ett detaljerat protokoll för att programmera humana perifera mononukleära blodceller (PBMNCs) som erhållits från frysta gula hinnor till virusfria iPSCs använder icke-integrerande episomala plasmider.

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Utvecklingsbiologi stamcellsbiologi cellbiologi molekylärbiologi inducerade pluripotenta stamceller perifera mononukleära blodceller omprogrammering episomala plasmider.
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