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JoVE Journal
Developmental Biology

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通过在胚胎梅克尔软骨Zebrabow克隆细胞分析软骨插层和定向扩散的可视化
 
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通过在胚胎梅克尔软骨Zebrabow克隆细胞分析软骨插层和定向扩散的可视化

Article DOI: 10.3791/52935-v 06:40 min October 21st, 2015
October 21st, 2015

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

颅面骨细胞组织长期以来一直推测但从未直接显现。多光谱细胞标记体内活 成像可以在斑马鱼下颌动态细胞行为的可视化。在这里,我们详细的协议来操作Zebrabow转基因鱼,直接观察细胞嵌入和麦克尔软骨细胞的形态变化。

Tags

发育生物学,第104,颅面发育,颅神经嵴,共聚焦显微镜,命运映射,多光谱克隆分析,SOX10,Zebrabow,斑马鱼,下颌,麦克尔软骨
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