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Developmental Biology

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Visualisering af Chondrocyt Intercalation og Directional spredning via Zebrabow Klonal Cell Analysis i Embryonale Meckel s Brusk
 
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Visualisering af Chondrocyt Intercalation og Directional spredning via Zebrabow Klonal Cell Analysis i Embryonale Meckel s Brusk

Article doi: 10.3791/52935
October 21st, 2015

Summary October 21st, 2015

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Celle organisering af kraniofaciale knogler har længe været en hypotese, men aldrig direkte visualiseres. Multispektral cellemærkning og in vivo levende billeddannelse tillader visualisering af dynamiske celle adfærd i zebrafisk underkæbe. Her har vi detaljeret protokollen til at manipulere Zebrabow transgene fisk og direkte observere celle intercalation og morfologiske ændringer af chondrocytter i Meckel s brusk.

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