Journal
/
Biology
/
Selected Reaction Monitoring spectrométrie de masse pour Absolute quantification des protéines
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Please note that all translations are automatically generated. Click here for the English version.

Selected Reaction Monitoring spectrométrie de masse pour Absolute quantification des protéines

français

Automatically Generated

16,836 Views

•

09:04 min

•

August 17, 2015

DOI:

10.3791/52959-v

DOI:
10.3791/52959-v

•

09:04 min
August 17, 2015

•

6 Views
, ,
1Cellular Networks Proteomics Unit, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Summary

Automatically generated

This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.

Explore More Videos

Absolute Protein QuantificationSelected Reaction MonitoringMass SpectrometryQuantotypic PeptidesProteotypic PeptidesPost-translational ModificationIsotope DilutionChemotaxis SignalingRAW 264.7 Cells

Read Article

Cite this Article

Manes, N. P., Mann, J. M., Nita-Lazar, A. Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification. J. Vis. Exp. (102), e52959, doi:10.3791/52959 (2015).

Download .ris file

Copy

Share Video

.

Copy