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Reacción seleccionada Monitoreo espectrometría de masas para Absolute Proteína cuantificación
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JoVE Journal Biology
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Reacción seleccionada Monitoreo espectrometría de masas para Absolute Proteína cuantificación

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16,846 Views

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09:04 min

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August 17, 2015

DOI:

10.3791/52959-v

DOI:
10.3791/52959-v

•

09:04 min
August 17, 2015

•

6 Views
, ,
1Cellular Networks Proteomics Unit, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Summary

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This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.

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Absolute Protein QuantificationSelected Reaction MonitoringMass SpectrometryQuantotypic PeptidesProteotypic PeptidesPost-translational ModificationIsotope DilutionChemotaxis SignalingRAW 264.7 Cells

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Cite this Article

Manes, N. P., Mann, J. M., Nita-Lazar, A. Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification. J. Vis. Exp. (102), e52959, doi:10.3791/52959 (2015).

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