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Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
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Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

Article DOI: 10.3791/52963-v • 12:30 min • August 17th, 2015
August 17th, 2015 •
1, 1,2, 1, 1, 1, 1
1Molecular Neurobiology Laboratory, Psychiatry, McLean Hospital, 2Department of Biochemistry, College of Medicine, Konyang University

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Summary
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Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.

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Nurr-1 Polyclonal Antibodies Cross-reactivity Nor1 Nur77 Nuclear Receptor Subfamily 4 DNA Binding Domain Ligand-binding Domain Dopaminergic System Dopamine Dysfunctions Parkinson's Disease Schizophrenia Manic Depression Hippocampus Learning And Memory Nerve Growth Factor IB Neuron-derived Orphan Receptor 1
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