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Immunology and Infection

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Cellule T Capture batteri da Transinfection da cellule dendritiche

Article DOI: 10.3791/52976-v • 11:39 min • January 13th, 2016
January 13th, 2016 •
1, 1,2, 1,2, 1,2, 1
1Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa)

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Qui un protocollo viene presentato per misurare la cattura batterica da parte delle cellule T CD4 + che si verifica durante la presentazione dell'antigene via transinfection dalle cellule dendritiche pre-infettati (DC). Mostriamo come eseguire i passi necessari: isolamento delle cellule primarie, infezione di DC, DC / T formazione coniugato cellulare, e la misura di batteri trasfezione delle cellule T.

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Immunologia Numero 107 transinfection presentazione dell'antigene le cellule T le cellule dendritiche batteri gentamicina test di sopravvivenza
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