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JoVE Journal Biology
Implementazione Patch Clamp e vivo microscopia a fluorescenza per il monitoraggio delle proprietà funzionali di recente isolato PKD Epithelium
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Implementazione Patch Clamp e vivo microscopia a fluorescenza per il monitoraggio delle proprietà funzionali di recente isolato PKD Epithelium

Article DOI: 10.3791/53035-v • 08:46 min • September 1st, 2015
September 1st, 2015 •
1, 1, 1, 1, 2, 1
1Department of Physiology, Medical College of Wisconsin, 2Department of Integrative Biology & Pharmacology, University of Texas Health Science Center at Houston

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Canali ionici espressi in renale tubulare svolgono un ruolo significativo nella patologia della malattia del rene policistico. Qui si descrive protocolli sperimentali utilizzati per eseguire patch-clamp analisi e di livello di calcio intracellulare misure in cistico appena isolato da reni di roditori.

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Medicina Numero 103 patch-clamp malattia del rene policistico ARPKD ADPKD rene calcio intracellulare Fura-02:00 nefrone sviluppo cisti policistina
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