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Journal / Biology / Implementering Patch Clamp og live Fluorescens…
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JoVE Journal Biology
Implementering Patch Clamp og live Fluorescens mikroskopi til Monitor funktionelle egenskaber af frisk isolerede PKD epitel
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Implementering Patch Clamp og live Fluorescens mikroskopi til Monitor funktionelle egenskaber af frisk isolerede PKD epitel

Article DOI: 10.3791/53035-v • 08:46 min • September 1st, 2015
September 1st, 2015 •
1, 1, 1, 1, 2, 1
1Department of Physiology, Medical College of Wisconsin, 2Department of Integrative Biology & Pharmacology, University of Texas Health Science Center at Houston

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Ionkanaler udtrykt i tubulær epitel spiller en væsentlig rolle i patologien af ​​polycystisk nyresygdom. Her beskriver vi forsøgsprotokoller bruges til at udføre patch-clamp analyse og intracellulært calcium niveau målinger i cystisk epitel frisk isoleret fra gnaver nyrerne.

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Medicin Patch-clamp polycystisk nyresygdom ARPKD ADPKD nyre intracellulær calcium Fura-2 AM nephron cyste udvikling polycystin
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