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Journal / Bioengineering / A Microfluidic Platform for Precision…
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Bioengineering

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JoVE Journal Bioengineering
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Article DOI: 10.3791/53051-v • 11:32 min • November 23rd, 2015
November 23rd, 2015 •
1,2, 1, 1, 1, 1, 1, 1, 1
1Materials Engineering Division, Lawrence Livermore National Laboratory, 2Department of Biomedical Engineering, Boston University

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Summary
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This protocol describes a system architecture for performing automated small volume (0.15–1.5 ml) particle separations using a microfluidic device, and discusses methods to optimize acoustofluidic device performance and operation.

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Microfluidic Platform Precision Small-volume Sample Processing Acoustic Microdevice Sample Manipulation Reagent Waste Reduction Integrated Microfluidic Platform Automated Fluid Handling World-to-chip Connections Modular Device Layout Robust Fixtures Closed-loop Sample Collection System Cleaning Priming Steps Acoustofluidic Device Characterization Performance Optimization Size-separation Of Biological Samples Real-time Feedback Sample Conservation And Concentration Equipment Integration
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